牦牛和柴达木黄牛低氧通气反应及颈动脉体NO、NOS的比较研究

doi:10.11843/j.issn.0366-6964.2018.10.024

Abstract

Abstract:

The aim of the present study to compare the hypoxic ventilatory response (HVR) characteristic and the content of nitric oxide (NO), nitric oxide synthase (NOS) in carotid body (CB) between yaks and Qaidam yellow cattle. In this study, health adult yaks and Qaidam yellow cattle at altitude of 3 200 m were inhaled hypoxic gas of 13.9% O₂, which was simulated to the altitude of 6 000 m; NO and NOS mRNA and NOS protein levels were detected in carotid body of yak and Qaidam yellow cattle living at altitude 3 200 m and simulate-altitude 6 000 m by the real-time quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and immunohistochemical methods. The results showed that the slopes of HVR (△V_E/△SaO₂) in yaks and Qaidam yellow cattle were (0.21±0.10) and (0.50±0.21) (L·min^-1)/% SaO₂ (P<0.01), respectively; There were no significant differences in the expression of the mRNA and protein of nNOS, eNOS, iNOS in CB between yak and Qaidam yellow cattle at altitude 3 200 m (P>0.05), in the expression of the protein of nNOS, eNOS, iNOS in CB between yak and Qaidam yellow cattle at simulate-altitude 6 000 m (P>0.05), in the expression of the protein of nNOS, eNOS, iNOS in yak CB between simulate-altitude 6 000 m and altitude 3 200 m, nor in Qaidam yellow cattle. The NO of the CB in yak and Qaidam yellow cattle at simulate-altitude 6 000 m was significantly higher than that of at altitude 3 200 m (P<0.01,P<0.05), respectively. The content of NO in yak CB was significantly higher than that of Qaidam yellow cattle at simulate-altitude 6 000 m (P<0.01). No significant difference in the content of NO in CB between yaks and Qaidam yellow cattle at altitude 3 200 m (P>0.05). The results suggested that HVR was blunted in Yak in Qinghai-Tibetan Plateau, and there was higher ventilatory response to hypoxia in Qaidam yellow cattles, NO production in the yak CB increases during acute hypoxia, and the elevated levels of NO may suppresses the CB response to hypoxia.

CLC Number:

S852.1

ZHANG Shou, JIN Guo-en, CHANG Lan,Lü Jun, SHEN Ming-hua, LIU Hui, GU Hai-yan, LEI Nai-hu. Comparison on Hypoxic Ventilatory Response and NO, NOS of Carotid Body between Yaks and Qaidam Yellow Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2276-2282.

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Comparison on Hypoxic Ventilatory Response and NO, NOS of Carotid Body between Yaks and Qaidam Yellow Cattle

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